| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAP3K7; TAK1; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1 |
| Entrez GeneID | 6885 |
| WB Predicted band size | Calculated MW: 67 kDa; Observed MW: 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser439 of human TAK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于 **Phospho-TAK1 (Ser439) 抗体**的参考文献示例(内容基于领域内典型研究方向，供参考)：
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1. **文献名称**：*TAK1 phosphorylation by AMPK promotes non-canonical NF-κB activation in response to metabolic stress*
**作者**：Liu H, et al.
**摘要**：研究揭示了TAK1在Ser439位点的磷酸化如何通过AMPK信号通路调控非经典NF-κB通路，从而在细胞代谢应激中促进炎症反应。文章使用Phospho-TAK1 (Ser439)抗体验证了该位点的磷酸化对TAK1活性的影响。
2. **文献名称**：*Phosphorylation of TAK1 at Ser439 regulates TGF-β-mediated epithelial-mesenchymal transition in breast cancer*
**作者**：Chen Y, et al.
**摘要**：通过免疫印迹和免疫荧光实验，该研究证明TAK1的Ser439磷酸化是TGF-β诱导乳腺癌细胞上皮-间质转化的关键步骤，Phospho-TAK1 (Ser439)抗体被用于检测该修饰在肿瘤转移中的作用。
3. **文献名称**：*Targeting TAK1 phosphorylation in rheumatoid arthritis: A novel therapeutic strategy*
**作者**：Wang X, et al.
**摘要**：文章探讨了抑制TAK1 Ser439磷酸化对类风湿关节炎模型炎症反应的缓解作用，通过该抗体确认了TAK1活性与关节病理进展的相关性。
4. **文献名称**：*Site-specific phosphorylation of TAK1 modulates its interaction with TAB1 in innate immunity*
**作者**：Kawai T, et al.
**摘要**：研究利用Phospho-TAK1 (Ser439)抗体揭示了该位点磷酸化对TAK1-TAB1复合物形成的调控机制，及其在TLR信号通路中的功能意义。
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**注**：以上文献为示例，实际引用时需根据具体研究通过PubMed或Google Scholar检索真实文献。
**Background of Phospho-TAK1 (Ser439) Antibody**
TAK1 (Transforming Growth Factor-β-Activated Kinase 1) is a serine/threonine kinase in the MAPK kinase family, playing a central role in mediating signals from cytokines, Toll-like receptors, and stress stimuli. It activates downstream pathways like NF-κB and MAPK, regulating processes such as inflammation, apoptosis, and immune responses. Phosphorylation at specific residues, including Ser439. is critical for TAK1 activation. The Ser439 site lies within the kinase domain, and its phosphorylation (often induced by upstream signals like TNF-α, IL-1. or LPS) promotes TAK1 autophosphorylation and interaction with adaptor proteins, enabling downstream signaling.
The Phospho-TAK1 (Ser439) antibody is a selective tool designed to detect TAK1 only when phosphorylated at Ser439. making it invaluable for studying TAK1 activation dynamics. Researchers use this antibody in techniques like Western blotting, immunoprecipitation, or immunofluorescence to investigate TAK1's role in diseases linked to dysregulated signaling, such as cancer, autoimmune disorders, and neurodegenerative conditions. Its specificity helps dissect signaling cascades in response to stimuli or therapeutic interventions, aiding drug development targeting TAK1-related pathways.
Validation typically includes testing in knockout models or phosphorylation-blocking assays to confirm specificity. This antibody is essential for understanding TAK1's regulatory mechanisms and its implications in health and disease.
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