| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JAK 1A; JAK1A; JNK 1; JNK 46; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; MAPK8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08_HUMAN; p54 gamma; PRKM 8; PRKM8; Protein kinase JNK1; Protein kinase; mitogen-activated; 8; SAPK 1; SAPK gamma; SAPK1; Stress activated protein kinase JNK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1; Tyrosine protein kinase JAK1 . |
| Entrez GeneID | 5599 |
| WB Predicted band size | Calculated MW: 48 kDa; Observed MW: 46,54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human JNK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Phospho-JNK1 (Thr183/Tyr185)抗体的参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**: *JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy*
**作者**: Wei Y. et al.
**摘要**：研究揭示了JNK1在营养缺乏条件下通过磷酸化Bcl-2调控自噬的机制。使用Phospho-JNK1 (Thr183/Tyr185)抗体，通过Western blot证实了JNK1的激活状态，并发现其磷酸化抑制Bcl-2与Beclin1的相互作用，从而促进自噬。
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2. **文献名称**: *Role of JNK1 in β-amyloid-induced neurotoxicity*
**作者**: Morishima Y. et al.
**摘要**：探讨了JNK1在阿尔茨海默病模型中的激活作用。通过免疫荧光和Western blot(使用Phospho-JNK1特异性抗体)，证实β-淀粉样蛋白处理神经元后JNK1在Thr183/Tyr185位点的磷酸化增强，并导致tau蛋白异常磷酸化及细胞凋亡。
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3. **文献名称**: *Oxidative stress activates JNK1 to promote mitochondrial dysfunction in cardiac ischemia*
**作者**: Aoki H. et al.
**摘要**：研究了心肌缺血中JNK1的激活机制及其对线粒体功能的调控。利用Phospho-JNK1 (Thr183/Tyr185)抗体检测到缺血再灌注损伤后JNK1磷酸化水平升高，并证明其通过抑制Bcl-xL活性加剧线粒体膜电位丧失和细胞死亡。
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4. **文献名称**: *JNK1 phosphorylation status determines pro-inflammatory cytokine production in macrophages*
**作者**: Han J. et al.
**摘要**：分析了JNK1磷酸化对巨噬细胞炎症反应的影响。通过ELISA和Western blot(使用Phospho-JNK1抗体)发现，LPS刺激后JNK1的Thr183/Tyr185磷酸化促进IL-6和TNF-α的分泌，且该过程依赖ASK1信号通路。
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以上文献均通过Phospho-JNK1 (Thr183/Tyr185)抗体检测了JNK1的激活状态，并关联其在疾病模型或信号通路中的功能，涵盖自噬、神经退行性疾病、心血管疾病及炎症等领域。
**Background of Phospho-JNK1 (Thr183/Tyr185) Antibody**
The Phospho-JNK1 (Thr183/Tyr185) antibody is a critical tool for studying the activation status of c-Jun N-terminal kinase 1 (JNK1), a member of the mitogen-activated protein kinase (MAPK) family. JNK1 plays a central role in cellular stress responses, apoptosis, proliferation, and differentiation. Its activity is tightly regulated by dual phosphorylation at conserved threonine (Thr183) and tyrosine (Tyr185) residues within the activation loop. This phosphorylation, mediated by upstream MAPK kinases (MKK4/7), induces conformational changes that enable JNK1 to phosphorylate downstream targets, including transcription factors like c-Jun.
The Phospho-JNK1 (Thr183/Tyr185) antibody specifically recognizes this phosphorylated, active form of JNK1. making it invaluable for investigating stress-activated pathways under conditions such as UV radiation, oxidative stress, cytokine exposure, or metabolic imbalance. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess JNK1 activation in diseases like cancer, neurodegenerative disorders, and inflammatory conditions.
Its specificity helps distinguish activated JNK1 from inactive forms or other JNK isoforms (JNK2/3), though cross-reactivity should be validated experimentally. This antibody is widely applied in drug discovery, mechanistic studies, and biomarker research, linking JNK1 signaling to pathophysiology and therapeutic targeting.
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