首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰蛋白酶(trypsin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PRSS1含量。PRSS1即胰蛋白酶原 1，是胰液中重要消化酶。研究发现，其基因突变可使酶活性异常，致胰腺炎发生。深入了解它与胰腺炎的关联机制，如异常激活途径、对胰腺组织损伤机制等，或有助于开发新的诊断方法和治疗策略。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，该产品主要服务于基础医学研究领域，如胰腺疾病机制探索、消化系统病理模型构建、炎症相关分子通路分析，以及药物开发过程中胰蛋白酶活性调控的疗效评估。科研人员可通过动态监测疾病模型或实验干预下胰蛋白酶的表达趋势，为揭示其生理病理功能提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alpha-trypsin chain 2 ELISA Kit; Beta-trypsin ELISA Kit; Cationic trypsinogen ELISA Kit; Digestive zymogen ELISA Kit; Nonfunctional trypsin 1 ELISA Kit; Prss1 ELISA Kit; Serine protease 1 ELISA Kit; TCR V beta 4.1 ELISA Kit; TRP1 ELISA Kit; TRY1 ELISA Kit; TRY1_HUMAN ELISA Kit; TRY4 ELISA Kit; TRYP1 ELISA Kit; Trypsin I ELISA Kit; Trypsinogen 1 ELISA Kit; Trypsinogen A ELISA Kit
缩写：PRSS1
Uniprot No.：P07477
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:2000 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:4000 | Average % | 89 | ||||
| Range % | 82-97 | |||||
| 1:8000 | Average % | 96 | ||||
| Range % | 91-108 | |||||
回收率：
| The recovery of human trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-99 | ||||
| EDTA plasma (n=4) | 96 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 40002.9012.8842.8932.759 20002.4912.4812.4862.352 10001.7361.7561.7461.612 5000.9420.9550.9490.815 2500.5070.5110.5090.375 1250.4620.4730.4680.334 62.50.2580.2520.2550.121 00.1320.1360.134 | ||||||
货期：3-5 working days
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