| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X |
| Entrez GeneID | 3014 |
| WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser139 of human gamma H2A.X |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-Histone H2A.X (Ser139)抗体的3篇关键参考文献：
1. **"DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139"**
- **作者**: Rogakou, E.P. et al. (1998)
- **摘要**: 该研究首次报道DNA双链断裂(DSBs)会触发组蛋白H2AX在Ser139位点的快速磷酸化(γ-H2AX)，并证实其可作为DSBs的分子标志物，为后续抗体开发奠定理论基础。
2. **"Megabase chromatin domains involved in DNA double-strand breaks in vivo"**
- **作者**: Rogakou, E.P. et al. (1999)
- **摘要**: 研究通过免疫荧光技术结合Phospho-H2A.X (Ser139)抗体，证明γ-H2AX在受损染色质区域形成聚焦点(foci)，为DNA损伤可视化检测提供关键实验依据。
3. **"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage"**
- **作者**: Matsuoka, S. et al. (2007)
- **摘要**: 利用Phospho-H2A.X抗体揭示ATM/ATR激酶在DNA损伤应答中调控γ-H2AX的分子机制，并构建了H2AX磷酸化在信号通路中的网络图谱。
4. **"γH2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and cell lines"**
- **作者**: Ivashkevich, A. et al. (2012)
- **摘要**: 该文献验证了Phospho-H2A.X (Ser139)抗体在定量检测电离辐射诱导的DNA损伤中的应用，证明其敏感性和特异性适用于临床前研究。
这些文献涵盖了γ-H2AX的生物学功能、检测方法及抗体应用场景，可作为相关实验的经典参考文献。
Phospho-Histone H2A.X (Ser139) antibody detects the phosphorylated form of histone H2A.X at serine 139. a well-established biomarker of DNA double-strand breaks (DSBs). Histone H2A.X, a variant of the H2A histone family, becomes rapidly phosphorylated at Ser139 (forming γ-H2A.X) in response to DNA damage, particularly DSBs induced by ionizing radiation, chemotherapeutic agents, or endogenous oxidative stress. This phosphorylation event is mediated by kinases such as ATM, ATR, and DNA-PK, which are activated as part of the DNA damage response (DDR) signaling cascade.
The γ-H2A.X foci serve as platforms to recruit DNA repair proteins (e.g., BRCA1. 53BP1) and chromatin remodelers, facilitating localized repair and maintaining genomic stability. The antibody is widely used in research to visualize and quantify DSBs, assess cellular responses to genotoxic stress, and study mechanisms underlying cancer, aging, and neurodegenerative diseases. It is a critical tool in immunofluorescence, Western blotting, and flow cytometry to evaluate DNA damage in preclinical models, drug screening, and radiation biology. Additionally, γ-H2A.X levels correlate with treatment efficacy, making this antibody valuable in evaluating the impact of radiotherapy or chemotherapy. Its specificity for the phosphorylated epitope ensures accurate detection of early DDR activation, aiding in mechanistic studies of genome integrity pathways.
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