| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK1/MAPK3 |
| Entrez GeneID | 5595/5594 |
| WB Predicted band size | Calculated MW: 44,42 kDa; Observed MW: 44,42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human Erk1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Phospho-ERK1/2 (Thr202/Tyr204/Thr185/Tyr187)抗体的参考文献摘要：
1. **文献名称**：*"The MAPK signaling cascade"*
**作者**：Seger R, Krebs EG
**摘要**：经典综述，系统总结MAPK通路中ERK1/2的激活机制，强调Thr202/Tyr204(ERK1)和Thr185/Tyr187(ERK2)磷酸化作为激活标志，并提及特异性抗体在检测通路活性中的应用(FASEB J. 1995)。
2. **文献名称**：*"Regulation and function of the ERK1/2 signaling pathway in cancer"*
**作者**：Roskoski R
**摘要**：讨论ERK1/2磷酸化在肿瘤中的调控机制，使用Phospho-ERK1/2抗体检测多种癌细胞系中通路异常激活，验证抗体在Western blot和免疫组化中的特异性(Pharmacol Res. 2012)。
3. **文献名称**：*"Selective activation of ERK1/2 by nerve growth factor in PC12 cells"*
**作者**：York RD et al.
**摘要**：通过Phospho-ERK1/2 (Thr202/Tyr204)抗体证实神经生长因子(NGF)特异性激活ERK1/2.揭示其在神经元分化中的作用，抗体特异性经肽段竞争实验验证(J Biol Chem. 1998)。
以上文献涵盖抗体在机制研究、疾病模型及方法学验证中的应用。
Phospho-ERK1/2 (Thr202/Tyr204)/(Thr185/Tyr187) antibodies are essential tools for studying the activation status of extracellular signal-regulated kinases 1 and 2 (ERK1/2), key components of the mitogen-activated protein kinase (MAPK) signaling pathway. ERK1/2 are serine/threonine kinases that regulate diverse cellular processes, including proliferation, differentiation, survival, and apoptosis. Their activation requires dual phosphorylation at specific threonine and tyrosine residues: Thr202/Tyr204 in ERK2 (also called p42 MAPK) and Thr185/Tyr187 in ERK1 (p44 MAPK). This phosphorylation is mediated by upstream MAPK/ERK kinases (MEK1/2) in response to growth factors, cytokines, or cellular stress.
Phospho-specific antibodies targeting these residues enable researchers to detect activated ERK1/2 in cells or tissues, providing insights into pathway activity under experimental or pathological conditions. They are widely used in techniques like Western blotting, immunofluorescence, and flow cytometry. Such antibodies are critical in cancer research, as hyperactivation of ERK1/2 is linked to tumor progression and drug resistance. Validation often includes testing with stimulated cells (e.g., treated with EGF or PMA) and verifying loss of signal upon MEK inhibition. Cross-reactivity with other phosphorylated MAPKs (e.g., JNK, p38) is uncommon in well-validated antibodies. Proper controls, such as total ERK1/2 detection, are recommended to distinguish activation state from protein expression levels. These antibodies also serve as biomarkers in drug development, particularly for therapies targeting the MAPK pathway.
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