| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RPS6KA3; ISPK1; MAPKAPK1B; RSK2; Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; p90RSK3; Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated |
| Entrez GeneID | 6197 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 84 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser227 of human Rsk 2/MAPKAP Kinase 1b |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与Phospho-RSK2 (Ser227)抗体相关的参考文献(模拟虚构，供参考)：
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1. **文献名称**: *"ERK-dependent phosphorylation of RSK2 on Ser227 regulates its subcellular localization and transcriptional activity"*
**作者**: Smith J, et al.
**摘要**: 研究揭示了ERK信号通路通过磷酸化RSK2的Ser227位点调控其核转位机制，使用Phospho-RSK2 (Ser227)抗体验证了生长因子刺激后该位点的磷酸化动态。
2. **文献名称**: *"RSK2-mediated c-Fos activation in response to oxidative stress requires phosphorylation at Ser227"*
**作者**: Lee H, et al.
**摘要**: 本文通过Western blot和免疫荧光技术，利用Phospho-RSK2 (Ser227)特异性抗体，证明氧化应激通过p38 MAPK/RSK2通路激活c-Fos，Ser227磷酸化是关键调控步骤。
3. **文献名称**: *"Targeting RSK2 phosphorylation in breast cancer models reveals a role in metastasis suppression"*
**作者**: Chen R, et al.
**摘要**: 研究使用Phospho-RSK2 (Ser227)抗体分析乳腺癌细胞中RSK2活性，发现抑制Ser227磷酸化可降低侵袭能力，提示其作为治疗靶点的潜力。
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(注：以上文献为模拟示例，实际引用需查询PubMed等数据库获取真实研究。)
The Phospho-RSK2 (Ser227) antibody is a specialized tool used to detect the activated form of Ribosomal S6 Kinase 2 (RSK2), a serine/threonine kinase belonging to the RSK family. RSK2 plays a critical role in cell survival, proliferation, and growth by integrating signals from the MAPK/ERK pathway. Its activation typically occurs through sequential phosphorylation events: ERK1/2 first phosphorylates RSK2 at Thr577. enabling autophosphorylation at Ser227 within the kinase domain. Phosphorylation at Ser227 is essential for RSK2’s catalytic activity, allowing it to phosphorylate downstream targets like CREB, NF-κB, and histone H3. thereby regulating gene expression and cellular responses.
This antibody specifically recognizes RSK2 when phosphorylated at Ser227. making it valuable for studying RSK2 activation in response to growth factors, hormones, or stress signals. Researchers use it in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess RSK2 activity in cell lines, tissues, or disease models. Dysregulated RSK2 signaling is implicated in cancers, neurological disorders (e.g., Coffin-Lowry syndrome), and metabolic diseases, underscoring the antibody’s relevance in both basic research and therapeutic development. By detecting Ser227 phosphorylation, this antibody helps elucidate RSK2’s role in signaling networks and its potential as a biomarker or drug target.
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