| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rb2; P130 |
| Entrez GeneID | 5934 |
| WB Predicted band size | Calculated MW: 128 kDa; Observed MW: 128 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser952 of human Rb2 p130 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于Phospho-Rb2 p130 (Ser952)抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
---
1. **文献名称**：*"Phosphorylation of p130Cas at Ser952 regulates cell cycle progression in response to DNA damage"*
**作者**：Smith A, et al.
**摘要**：研究揭示了DNA损伤后，Ser952位点的磷酸化通过调控p130与E2F4的相互作用，影响G1/S期检查点，该抗体用于检测磷酸化状态以验证细胞周期停滞机制。
2. **文献名称**：*"Role of p130Cas phosphorylation in breast cancer metastasis"*
**作者**：Zhang Y, et al.
**摘要**：探讨了Ser952磷酸化在乳腺癌细胞迁移中的作用，利用该抗体证明其与AKT信号通路激活相关，并关联肿瘤侵袭性表型。
3. **文献名称**：*"Cell cycle-dependent regulation of p130 by CDK2 kinase"*
**作者**：Mori T, et al.
**摘要**：通过免疫印迹分析(使用Phospho-Rb2 p130 Ser952抗体)，证实CDK2介导的Ser952磷酸化促进p130降解，从而驱动细胞从G1期进入S期。
---
注：以上文献为示例，实际引用需根据具体研究补充真实来源。若需精准文献，建议通过PubMed或Google Scholar以关键词检索，并筛选抗体供应商(如CST、Abcam)提供的引用文献列表。
The Phospho-Rb2 p130 (Ser952) antibody is a specialized tool used to detect the phosphorylated form of the retinoblastoma-like protein p130 (RBL2) at serine residue 952. Rb2/p130. a member of the retinoblastoma tumor suppressor family (including Rb/p105. p107. and p130), plays a critical role in cell cycle regulation by interacting with E2F transcription factors to repress cell cycle progression, particularly in the G0/G1 phase. Phosphorylation of p130 at specific residues, such as Ser952. modulates its function by disrupting its binding to E2F, thereby releasing E2F-mediated transcriptional repression and allowing cell cycle entry. This phosphorylation event is typically mediated by cyclin-dependent kinases (CDKs) during cell cycle transitions, especially in response to mitogenic signals.
The antibody is widely used in cancer research to study aberrant cell cycle regulation, as dysregulation of p130 phosphorylation is implicated in tumorigenesis. It helps assess the activation status of p130 in various cellular contexts, including differentiation, senescence, and apoptosis. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate cell cycle dynamics, therapeutic responses to CDK inhibitors, or correlations between p130 phosphorylation and disease progression. Its specificity for the phosphorylated Ser952 epitope makes it valuable for distinguishing active from inactive p130 in experimental models, contributing to understanding tumor suppressor mechanisms and developing targeted therapies.
×
