| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Entrez GeneID | 5925 |
| WB Predicted band size | Calculated MW: 106 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser780 of human Rb |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于Phospho-Rb (Ser780)抗体的3篇参考文献示例(文献信息为虚构，仅供示例参考)：
1. **"CDK4/6-mediated phosphorylation of RB at Ser780 drives cell cycle progression"**
*作者：Harper, J.W. et al.*
摘要：研究CDK4/6激酶对Rb蛋白Ser780位点的磷酸化作用，通过Phospho-Rb (Ser780)抗体验证其在G1/S期转换中的关键功能，并揭示该位点磷酸化与E2F转录激活的关联。
2. **"Validation of a phosphospecific antibody targeting Retinoblastoma protein at Serine 780"**
*作者：Chen, H. et al.*
摘要：通过Western blot和免疫荧光实验验证Phospho-Rb (Ser780)抗体的特异性，证明其在检测CDK4/6抑制剂处理后的细胞中Rb磷酸化水平变化的高敏感性。
3. **"Dysregulated RB phosphorylation at Ser780 correlates with poor prognosis in breast cancer"**
*作者：Mendoza, M. et al.*
摘要：利用Phospho-Rb (Ser780)抗体对乳腺癌组织进行免疫组化分析，发现Ser780磷酸化水平升高与肿瘤侵袭性及患者生存率下降显著相关。
4. **"Mechanistic insights into RB phosphorylation dynamics during DNA damage response"**
*作者：Smith, L.M. et al.*
摘要：探讨DNA损伤条件下Rb蛋白Ser780位点的磷酸化调控机制，结合Phospho-Rb (Ser780)抗体揭示其与细胞周期停滞的分子关联。
(注：以上文献为模拟生成，实际引用时请通过PubMed或学术数据库检索真实文献。)
The Phospho-Rb (Ser780) antibody is a tool used to detect the retinoblastoma protein (Rb) when phosphorylated at serine residue 780. a key post-translational modification involved in cell cycle regulation. Rb, a tumor suppressor encoded by the *RB1* gene, plays a critical role in controlling the G1-to-S phase transition by binding and inhibiting E2F transcription factors. Phosphorylation of Rb by cyclin-dependent kinases (CDKs), particularly CDK4/6-cyclin D complexes, at specific residues (including Ser780) disrupts its interaction with E2F, allowing cell cycle progression.
The Ser780 phosphorylation site is a well-characterized marker of CDK4/6 activity and is commonly used to assess Rb inactivation in cancer and cell proliferation studies. Dysregulation of Rb phosphorylation is implicated in tumorigenesis, as hyperphosphorylation (and subsequent functional loss of Rb) is observed in many cancers. This antibody is widely employed in techniques like Western blotting, immunohistochemistry, and flow cytometry to study cell cycle dynamics, drug responses (e.g., CDK4/6 inhibitors), and disease mechanisms.
Its specificity for the phosphorylated form of Rb makes it valuable in distinguishing active versus inactive Rb states, aiding research on therapeutic targeting, cell differentiation, and apoptosis. Validation typically includes testing in models with Rb phosphorylation modulation (e.g., CDK4/6 inhibition) to confirm signal specificity.
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