首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前颗粒蛋白(PGRN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的PGRN含量。试剂盒检测范围为4.7 ng/mL-300 ng/mL，支持科研人员通过定量检测PGRN在细胞模型中的分泌动态，或探索其在神经系统疾病、癌症等研究领域的生物标志物价值，也可用于评估药物干预或基因修饰对PGRN表达的影响，为分子机制研究提供可靠工具，适用于基础科研和体外生物医学研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PGRN
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：4.7 ng/mL-300 ng/mL
灵敏度：1.17 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 103 | ||||
| Range % | 97-110 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human PGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 89 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 3002.8242.9522.8882.717 1502.3322.1762.2542.083 751.4881.4041.4461.275 37.50.8560.8390.8480.677 18.750.4950.4740.4850.314 9.40.3350.3590.3470.176 4.70.2210.2290.2250.054 00.1640.1770.171 | ||||||
货期：3-5 working days
×
