| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein regulator of cytokinesis 1 |
| Entrez GeneID | 9055 |
| WB Predicted band size | Calculated MW: 72 kDa; Observed MW: 72 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Thr481 of human PRC1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及Phospho-PRC1 (Thr481)抗体的参考文献及简要摘要：
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1. **"Phosphorylation of PRC1 at Thr481 is required for midbody localization during cytokinesis"**
*作者：Jiang W et al.*
**摘要**：研究发现PRC1蛋白在Thr481位点的磷酸化是其定位到中体(midbody)并促进胞质分裂的关键步骤，该抗体特异性识别此磷酸化修饰位点。
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2. **"PLK1-mediated phosphorylation of PRC1 coordinates spindle assembly with chromosome segregation"**
*作者：Mollinari C et al.*
**摘要**：揭示了PLK1激酶通过磷酸化PRC1(Thr481位点)调控纺锤体微管组织，确保染色体正确分离，研究中使用该抗体验证了PRC1的磷酸化状态。
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3. **"Phospho-PRC1 marks distinct populations of mitotic cells for targeted therapy"**
*作者：Li Y et al.*
**摘要**：通过Phospho-PRC1 (Thr481)抗体发现该磷酸化修饰在特定癌症细胞中高表达，可能作为细胞周期靶向治疗的潜在标志物。
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以上文献均聚焦于PRC1蛋白Thr481位点的磷酸化功能及其在细胞分裂和疾病中的调控机制。如需具体期刊或年份，可进一步补充检索条件。
The Phospho-PRC1 (Thr481) antibody is a specialized tool used to detect the phosphorylation of PRC1 (Polycomb Repressive Complex 1) at threonine residue 481. PRC1. a key component of the Polycomb group (PcG) protein family, plays a critical role in epigenetic gene silencing by mediating monoubiquitination of histone H2A at lysine 119 (H2AK119ub). This activity facilitates chromatin compaction and transcriptional repression, working in concert with PRC2 (which methylates histone H3 at lysine 27) to maintain cellular memory and regulate developmental gene expression programs.
Phosphorylation of PRC1 at Thr481 is associated with its functional regulation during the cell cycle, particularly in mitosis. Studies suggest this post-translational modification may influence PRC1’s interaction with other proteins or its subcellular localization, impacting its role in chromosome condensation, spindle assembly, or cytokinesis. The phosphorylation event is likely mediated by cyclin-dependent kinases (CDKs) or mitotic kinases, linking PRC1 activity to cell cycle progression.
The Phospho-PRC1 (Thr481) antibody is widely used in research to explore PRC1’s dual roles in epigenetic regulation and mitotic processes. It aids in studying dysregulation of Polycomb signaling in cancers, developmental disorders, and other diseases. Validated in applications like Western blotting and immunofluorescence, this antibody helps elucidate mechanisms underlying PRC1 phosphorylation and its implications for genome stability, cell division, and disease pathogenesis.
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