| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ITGB2; CD18; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150; 95 subunit beta; Complement receptor C3 subunit beta; CD antigen CD18 |
| Entrez GeneID | 3689 |
| WB Predicted band size | Calculated MW: 85 kDa; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic phosphopeptide corresponding to residues surrounding Ser745 of human CD18 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
+ +
以下是关于Phospho-CD18 (Ser745)抗体的3篇参考文献示例(注：以下内容为模拟虚构，实际文献需通过数据库验证)：
---
1. **文献名称**: *"Phosphorylation of CD18 at Serine 745 regulates leukocyte adhesion and migration"*
**作者**: Smith A, et al.
**摘要**: 本研究通过Phospho-CD18 (Ser745)特异性抗体，揭示了CD18在炎症反应中Ser745位点的磷酸化对白细胞整合素活性及跨内皮迁移的关键调控作用，证明该修饰增强β2整合素与配体的结合能力。
---
2. **文献名称**: *"Ser745 phosphorylation of CD18 modulates neutrophil activation in sepsis"*
**作者**: Johnson B, et al.
**摘要**: 利用Phospho-CD18 (Ser745)抗体，作者发现脓毒症模型中中性粒细胞的CD18 Ser745磷酸化水平显著升高，该修饰通过激活MAPK/ERK通路促进中性粒细胞粘附和炎症因子释放。
---
3. **文献名称**: *"CD18 phosphorylation dynamics in T cell receptor signaling"*
**作者**: Lee C, et al.
**摘要**: 通过免疫印迹和流式细胞术结合Phospho-CD18 (Ser745)抗体，研究证明T细胞受体激活后CD18的Ser745磷酸化是调控免疫突触形成及T细胞活化的关键步骤。
---
如需真实文献，建议在PubMed或Google Scholar检索关键词：
`Phospho-CD18 Ser745 antibody`、`CD18 phosphorylation Ser745`、`integrin beta2 phosphorylation`。
部分抗体厂商(如Cell Signaling Technology)的技术文档也可能提供相关引用。
The Phospho-CD18 (Ser745) antibody detects the phosphorylated form of CD18 (integrin β2 subunit) at serine residue 745. a post-translational modification critical for regulating integrin activation and leukocyte function. CD18 is a key component of β2 integrins (e.g., LFA-1. Mac-1), which mediate leukocyte adhesion, migration, and immune responses by binding to endothelial cells or pathogens. Phosphorylation at Ser745. located in the cytoplasmic tail of CD18. is implicated in inside-out signaling, modulating integrin conformational changes and affinity for ligands like ICAM-1. This modification is tightly regulated by kinases/phosphatases in response to chemokines or antigens, influencing immune cell recruitment during inflammation, infection, or autoimmune diseases.
The antibody is widely used in research to study β2 integrin activation dynamics in neutrophils, macrophages, and lymphocytes. Applications include Western blotting, immunofluorescence, and flow cytometry to assess phosphorylation status in models of inflammatory disorders (e.g., rheumatoid arthritis, sepsis), immune deficiencies, or leukocyte adhesion deficiencies (LAD). By detecting Ser745 phosphorylation, it helps elucidate mechanisms underlying leukocyte extravasation, pathogen clearance, and chronic inflammation, offering insights for therapeutic targeting of integrin-mediated pathologies. Proper controls (e.g., unstimulated cells, phosphatase treatment) are essential to validate specificity due to transient nature of phosphorylation events.
×
