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产品描述：人过氧化物氧化还原酶-1(PRDX1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PRDX1含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于氧化应激相关机制研究、疾病模型中的抗氧化功能评估，以及药物干预后PRDX1表达水平监测等科研场景，为探索PRDX1在细胞保护、信号转导及病理生理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Heme binding 23 kDa protein ELISA Kit; MSP23 ELISA Kit; Natural killer cell-enhancing factor A ELISA Kit; NKEF A ELISA Kit; NKEF-A ELISA Kit; NKEFA ELISA Kit; OSF3 ELISA Kit; Osteoblast specific factor 3 ELISA Kit; PAG ELISA Kit; Paga ELISA Kit; PAGB ELISA Kit; Peroxiredoxin 1 ELISA Kit; Peroxiredoxin-1 ELISA Kit; PRDX1 ELISA Kit; PRDX1_HUMAN ELISA Kit; Proliferation associated gene A ELISA Kit; Proliferation-associated gene protein ELISA Kit; PRX1 ELISA Kit; PrxI ELISA Kit; TDPX2 ELISA Kit; Thioredoxin peroxidase 2 ELISA Kit; Thioredoxin-dependent peroxide reductase 2 ELISA Kit
缩写：PRDX1
Uniprot No.：Q06830
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-91 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human PRDX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 100 | 97-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.4622.4972.4802.2951001.7261.7361.7311.546501.1211.1641.1430.958250.6830.6980.6910.50612.50.4750.4980.4870.3026.250.3510.3670.3590.1743.120.2970.2870.2920.10700.1860.1830.185 | ||||||
货期：3-5 working days
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