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产品描述：人脂肪甘油三酯脂酶(ATGL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的PNPLA2含量。PNPLA2是一个备受关注的靶点。它主要参与脂肪代谢过程，对甘油三酯水解起着关键作用。相关研究聚焦于其调节脂肪降解的机制，探索通过调控该靶点来改善脂质代谢紊乱相关疾病，如肥胖、脂肪肝等。试剂盒检测范围为6.25 mIU/mL-400 mIU/mL，可广泛应用于脂肪代谢机制研究、能量稳态相关疾病模型（如非酒精性脂肪肝、2型糖尿病）的体外实验分析，以及药物干预下脂质代谢通路的动态监测，为代谢组学研究提供稳定可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：1110001C14Rik ELISA Kit; Adipose triglyceride lipase ELISA Kit; ATGL ELISA Kit; ATGL DESNUTRIN ELISA Kit; Calcium independent phospholipase A2 ELISA Kit; Calcium-independent phospholipase A2 ELISA Kit; Desnutrin ELISA Kit; EC 3.1.1.3 ELISA Kit; FP17548 ELISA Kit; IPLA2 zeta ELISA Kit; IPLA2-zeta ELISA Kit; Mutant patatin like phospholipase domain containing 2 ELISA Kit; Patatin like phospholipase domain containing 2 ELISA Kit; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 ELISA Kit; Patatin-like phospholipase domain-containing protein 2 ELISA Kit; PEDF R ELISA Kit; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA ELISA Kit; Pigment epithelium derived factor ELISA Kit; Pigment epithelium-derived factor ELISA Kit; plpl ELISA Kit; plpl2 ELISA Kit; PLPL2_HUMAN ELISA Kit; Pnpla2 ELISA Kit; Transport secretion protein 2 ELISA Kit; Transport secretion protein 2.2 ELISA Kit; Transport-secretion protein 2 ELISA Kit; Triglyceride hydrolase ELISA Kit; TTS 2.2 ELISA Kit; TTS2 ELISA Kit; TTS2.2 ELISA Kit; ZETA ELISA Kit
缩写：PNPLA2
Uniprot No.：Q96AD5
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates
检测范围：6.25 mIU/mL-400 mIU/mL
灵敏度：1.56 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ATGL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-110 | |||||
回收率：
| The recovery of human ATGL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 82-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected4002.0831.9922.0381.9162001.8541.7961.8251.7031001.5291.4841.5071.385501.0380.9761.0070.885250.7710.7090.7400.61812.50.4290.3950.4120.2906.250.2860.2690.2780.15600.1240.1190.122 | ||||||
货期：3-5 working days
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