首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人黑色素浓缩激素(PMCH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PMCH含量。PMCH即前黑素聚集激素，它是一种神经肽。在其与能量代谢、睡眠觉醒等生理过程相关。研究机制上，主要是通过与特定受体结合，激活细胞内信号通路来发挥作用，目前在肥胖、睡眠障碍等病症的治疗研究中受关注。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探索PMCH在能量代谢调控、神经内分泌系统中的作用机制，或用于评估疾病模型、药物干预实验中的PMCH动态变化。该产品严格适配科研场景，为代谢性疾病、神经科学等领域的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PMCH ELISA Kit; MCHPro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid ELISA Kit; NGE ELISA Kit; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine ELISA Kit; NEI ELISA Kit; Neuropeptide E-I); Melanin-concentrating hormone ELISA Kit; MCH)] ELISA Kit
缩写：PMCH
Uniprot No.：P20382
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 83-91 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of human PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 95-104 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.6822.5332.6082.4005002.0401.9461.9931.7852501.4191.3571.3881.1801250.9270.9750.9510.74362.50.6540.6890.6720.46431.20.4080.3920.4000.19215.60.3350.3240.3300.12200.2040.2110.208 | |||||||
货期：3-5 working days
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