| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SS1; DRB1; HLA-DRB; HLA-DR1B |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human HLA-DRB1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TriMethyl-Histone H3 (Lys79)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **Histone H3 Lysine 79 Methylation and the Response to DNA Double-Strand Breaks**
*作者：Huyen, Y. et al. (2004)*
**摘要**：研究发现H3K79me3在DNA双链断裂修复中起关键作用，通过抗体检测发现该修饰招募53BP1蛋白至损伤位点，揭示其与基因组稳定性调控的关联。
2. **DOT1L-Mediated H3K79 Methylation in MLL-Rearranged Leukemia**
*作者：Bernt, K.M. et al. (2011)*
**摘要**：利用H3K79me3抗体分析DOT1L抑制剂对白血病细胞的影响，证明H3K79异常甲基化促进MLL重排白血病的发生，为靶向治疗提供依据。
3. **Global Analysis of Histone Methylation Reveals H3K79 Patterns in Transcriptional Regulation**
*作者：Nguyen, A.T. & Zhang, Y. (2011)*
**摘要**：通过ChIP-seq和H3K79me3抗体分析，揭示H3K79甲基化在全基因组的分布特征及其在转录延伸和端粒沉默中的双重功能。
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以上文献均涉及H3K79me3抗体的应用，涵盖DNA修复、疾病机制及全基因组分析等领域。如需具体期刊或补充信息，可进一步检索PMID或DOI。
The TriMethyl-Histone H3 (Lys79) antibody specifically detects histone H3 trimethylated at lysine 79 (H3K79me3), a post-translational modification associated with transcriptional regulation and chromatin dynamics. H3K79 methylation occurs within the globular core of histone H3. a region less commonly modified compared to histone tails. This modification is catalyzed by the methyltransferase DOT1L in mammals, which is unique for methylating lysine residues in a non-tail histone region. H3K79me3 is enriched in transcriptionally active euchromatin and plays roles in DNA repair, cell cycle regulation, and gene silencing. It has also been implicated in maintaining genomic stability and proper chromosome segregation during mitosis.
Studies link aberrant H3K79me3 levels to diseases such as leukemia, where DOT1L overexpression due to chromosomal translocations drives oncogenic gene expression. The TriMethyl-Histone H3 (Lys79) antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to investigate epigenetic mechanisms in development, cancer, and stem cell biology. Its specificity for the trimethylated state (over mono- or dimethylation) ensures accurate detection of this modification’s functional role. Researchers rely on this antibody to explore how H3K79me3 dynamics contribute to disease pathways or cellular differentiation, making it a critical tool in epigenetics and translational research.
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