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产品描述：人丛状蛋白B1(PLXNB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PLXNB1含量。试剂盒检测范围为78 pg/mL-5000 pg/mL，该产品可广泛应用于科研领域，例如探究PLXNB1在神经退行性疾病中的调控机制、肿瘤微环境中的信号通路交互作用，或评估其在免疫细胞激活过程中的功能表达变化，为疾病机制研究及潜在治疗靶点探索提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PLXNB1 ELISA Kit; KIAA0407 ELISA Kit; PLXN5 ELISA Kit; SEP ELISA Kit; Plexin-B1 ELISA Kit; Semaphorin receptor SEP ELISA Kit
缩写：PLXNB1
Uniprot No.：O43157
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLXNB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
回收率：
| The recovery of human PLXNB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 96-107 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected50002.5432.4462.4952.38925001.6191.5341.5771.47112500.8720.9210.8970.7916250.5080.5200.5140.4083120.3350.3180.3270.2211560.2170.2050.2110.105780.1510.1590.1550.04900.1040.1070.106 | |||||||
货期：3-5 working days
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