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产品描述：人磷脂转运蛋白(PLTP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLTP含量。人磷脂转运蛋白(PLTP)是一种在肝脏和小肠中表达的脂质转运蛋白，主要参与高密度脂蛋白(HDL)的动态重塑，调节胆固醇逆转运过程。研究显示，PLTP在动脉粥样硬化等代谢性疾病中发挥重要作用，其功能机制涉及脂质代谢和免疫调节。试剂盒检测范围为2.34 ng/mL-150 ng/mL，适用于体外研究PLTP在脂质代谢调控、心血管疾病机制或肥胖相关信号通路中的功能，也可用于细胞模型、动物实验或临床前研究中的蛋白表达水平分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BPI fold containing family E ELISA Kit; BPIFE ELISA Kit; HDLCQ9 ELISA Kit; High density lipoprotein cholesterol level quantitative trait locus 9; included ELISA Kit; Lipid transfer protein II ELISA Kit; OD107 ELISA Kit; Phospholipid transfer protein ELISA Kit; Plasma phospholipid transfer protein ELISA Kit; Pltp ELISA Kit; PLTP_HUMAN ELISA Kit
缩写：PLTP
Uniprot No.：P55058
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：2.34 ng/mL-150 ng/mL
灵敏度：0.58 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLTP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 88-97 | |||||
| 1:200 | Average % | 104 | ||||
| Range % | 98-107 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 92-101 | |||||
| 1:800 | Average % | 99 | ||||
| Range % | 93-102 | |||||
回收率：
| The recovery of human PLTP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 93-103 | ||||
| EDTA plasma (n=4) | 97 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1502.6672.5472.6072.510751.8441.8311.8381.74137.51.0221.0171.0200.92318.750.5710.5820.5770.4809.3750.3280.3330.3310.2344.680.2540.2450.2500.1532.340.1560.1660.1610.06400.0960.0980.097 | ||||||
货期：3-5 working days
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