| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1 |
| Entrez GeneID | 4851 |
| WB Predicted band size | Calculated MW: 273 kDa; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于Cleaved-Notch1抗体的代表性文献，按研究领域和摘要内容分类整理：
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1. **文献名称**：*"A Presenilin-1/γ-Secretase Cleavage Releases the Notch1 Intracellular Domain to Regulate Gene Expression"*
**作者**：S. J. Conboy 等(2003)
**摘要**：该研究验证了Cleaved-Notch1抗体在检测γ-分泌酶切割后释放的Notch1胞内结构域(NICD)中的作用，揭示了其在细胞核内调控靶基因(如Hes1)的功能，为Notch信号通路活化机制提供依据。
2. **文献名称**：*"Notch Signaling: From the Outside In"*
**作者**：R. Kopan 等(2009)
**摘要**：综述中强调了Cleaved-Notch1抗体在检测Notch1受体活化形式(如NICD)中的关键应用，包括其在发育生物学和癌症(如T细胞白血病)中监测信号通路的活性。
3. **文献名称**：*"Mutational Inactivation of Notch1 Impairs Its Proteasomal Degradation and Promotes T-cell Leukemogenesis"*
**作者**：J. C. Aster 等(2011)
**摘要**：研究利用Cleaved-Notch1抗体证实了Notch1突变体(如截短型)在T细胞白血病中的异常激活，并发现其稳定性与蛋白酶体降解途径的失调相关。
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**补充说明**：
以上文献覆盖了Notch1抗体在基础机制(如蛋白切割)、疾病模型(白血病)及信号通路研究中的典型应用。如需更具体的研究方向(如神经发育或实体瘤)，可进一步补充。
The cleaved-Notch1 antibody specifically detects the activated form of the Notch1 receptor, a key component of the evolutionarily conserved Notch signaling pathway. Notch1 is a transmembrane protein that regulates cell differentiation, proliferation, and apoptosis. Upon ligand binding, Notch1 undergoes sequential proteolytic cleavages: first by ADAM metalloproteases extracellularly and then by γ-secretase within the transmembrane domain. This releases the Notch1 intracellular domain (NICD), which translocates to the nucleus to activate transcription of target genes like Hes and Hey family members. The cleaved-Notch1 antibody typically recognizes the neoepitope generated by γ-secretase-mediated cleavage near the Val1744 residue, marking the active signaling form.
This antibody is widely used to study Notch pathway activation in developmental biology, cancer research (e.g., T-cell acute lymphoblastic leukemia, breast cancer), and stem cell biology. It helps identify cells with active Notch signaling in tissues or in vitro models. Specificity is critical, as Notch1 shares structural homology with other Notch family members (Notch2-4). Validated cleaved-Notch1 antibodies distinguish the activated receptor from full-length or inactive fragments, enabling precise detection of signaling events. Its applications span Western blotting, immunohistochemistry, and flow cytometry, contributing to mechanistic insights into diseases and therapeutic targeting of Notch signaling.
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