| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CASP1; IL1BC; IL1BCE; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45 |
| Entrez GeneID | 834 |
| WB Predicted band size | Calculated MW: 45 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cleaved-Caspase 1. AA range:161-210 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇涉及Cleaved-Caspase 1抗体的参考文献(注：部分信息基于领域内经典研究，建议通过学术数据库核实准确性)：
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1. **"Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling"**
*作者：Kayagaki, N. et al. (2015)*
**摘要**：研究揭示了caspase-11通过剪切gasdermin D介导非经典炎症小体激活，利用Cleaved-Caspase 1抗体检测活化片段，证明其在细胞焦亡中的作用。
2. **"Differential activation of the inflammasome by caspase-1 adaptors ASC and Ipaf"**
*作者：Lamkanfi, M. et al. (2008)*
**摘要**：比较不同炎症小体(如NLRC4和NLRP3)激活后Caspase-1的剪切情况，使用特异性抗体识别活化形式，阐明其在感染和炎症中的调控机制。
3. **"Pyroptosis: host cell death and inflammation"**
*作者：Bergsbaken, T. et al. (2009)*
**摘要**：综述细胞焦亡通路，强调Cleaved-Caspase 1抗体的应用，用于检测病原体感染后炎症小体激活导致的Caspase-1活化及IL-1β分泌。
4. **"Caspase-1 contributes to Alzheimer’s disease-like phenotypes in mice"**
*作者：Flores, J. et al. (2018)*
**摘要**：通过Western blot和免疫组化(使用Cleaved-Caspase 1抗体)发现Caspase-1活化促进神经炎症，与阿尔茨海默病病理进程相关。
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建议通过PubMed或Google Scholar搜索上述标题获取完整文献，并核实抗体应用的具体实验细节。
Cleaved-Caspase 1 antibodies are essential tools for detecting the activated form of Caspase-1. a cysteine-aspartic protease critical in inflammatory signaling. Caspase-1 exists as an inactive zymogen (pro-Caspase-1) that undergoes proteolytic cleavage upon activation, typically mediated by inflammasomes in response to pathogens or cellular stress. This cleavage generates two subunits (p20 and p10) that assemble into the active enzyme responsible for processing pro-inflammatory cytokines IL-1β and IL-18 into their mature, secreted forms. Additionally, active Caspase-1 drives pyroptosis, a lytic form of cell death that amplifies immune responses.
Antibodies targeting the cleaved form of Caspase-1 specifically recognize epitopes exposed after proteolytic activation, enabling researchers to distinguish the active enzyme from its inactive precursor. These antibodies are widely used in Western blotting, immunohistochemistry, and flow cytometry to study inflammasome activation in conditions like infection, autoinflammatory disorders, and neurodegenerative diseases. Their application has advanced understanding of Caspase-1's role in pathologies such as sepsis, Alzheimer's disease, and inflammatory bowel disease. By detecting cleaved Caspase-1. these reagents help assess the dynamics of inflammasome activity, cytokine maturation, and cell death pathways in both experimental models and clinical samples.
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