首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠尿激酶型纤溶酶原激活物受体(PLAUR/uPAR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAUR含量。PLAUR即尿激酶型纤溶酶原激活物受体，在细胞表面参与调节纤溶、细胞迁移等过程。其背景上和多种生理病理活动相关，尤其是肿瘤进展。研究机制主要是激活纤溶酶原，促进细胞外基质降解，还能与多种信号通路相互作用，影响细胞增殖、侵袭等。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于探索PLAUR/uPAR在疾病模型中的表达调控机制、生物标志物筛选或药理学研究中的靶点验证。在肿瘤侵袭转移、炎症反应及纤维化疾病研究中具有重要价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PlaurUrokinase plasminogen activator surface receptor ELISA kit; U-PAR ELISA kit; uPAR ELISA kit; CD antigen CD87 ELISA kit
缩写：PLAUR
Uniprot No.：P49616
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PLAUR/uPAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 98-106 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 88-99 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 87-96 | ||||||
回收率：
| The recovery of rat PLAUR/uPAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 83-96 | |||||
| EDTA plasma (n=4) | 104 | 96-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.1132.2732.1932.07251.6431.7321.6881.5672.51.0721.1191.0960.9751.250.6820.6910.6870.5660.6250.4040.4180.4110.2900.3120.2760.2890.2830.1620.1560.2060.2030.2050.08400.1200.1210.121 | |||||||
货期：3-5 working days
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