首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠尿激酶型纤溶酶原激活物(uPA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAU含量。PLAU是一种重要的靶点。它参与多种生理病理过程，与肿瘤细胞的侵袭、转移密切相关。在研究机制方面，主要聚焦于其酶活性及信号通路，明确它对肿瘤微环境的调控作用，为开发针对性的抗肿瘤药物指明方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于心血管疾病、纤维化疾病或肿瘤相关动物模型中纤溶系统活性的动态监测，以及细胞迁移、组织修复等基础研究中的uPA表达水平分析，为科研人员探究uPA在病理生理过程中的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PlauUrokinase-type plasminogen activator ELISA kit; U-plasminogen activator ELISA kit; uPA ELISA kit; EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A; Urokinase-type plasminogen activator short chain A; Urokinase-type plasminogen activator chain B] ELISA kit
缩写：PLAU
Uniprot No.：P29598
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat uPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 88-95 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 94-103 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
回收率：
| The recovery of rat uPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-94 | |||||
| EDTA plasma (n=4) | 96 | 94-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 10002.3342.1862.2602.077 5001.6521.6251.6391.456 2501.0470.9971.0220.839 1250.6360.6880.6620.479 62.50.3980.4020.4000.217 31.20.3250.3370.3310.148 15.60.2460.2320.2390.056 00.1890.1770.183 | |||||||
货期：3-5 working days
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