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产品描述：人磷酸激酶1 (PGK1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PGK1含量。PGK1是一种糖酵解关键酶，参与肿瘤糖酵解途径，影响肿瘤生长。研究显示，PGK1可被肿瘤相关巨噬细胞调控，通过磷酸化促进肿瘤细胞糖酵解，进而促进肿瘤生长。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于体外培养细胞模型、动物组织或体液样本中PGK1的定量分析；为研究糖酵解调控机制、肿瘤代谢重编程、疾病相关生物标志物筛选等科研项目提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cell migration-inducing gene 10 protein ELISA Kit; Epididymis secretory sperm binding protein Li 68p ELISA Kit; HEL S 68p ELISA Kit; MGC117307 ELISA Kit; MGC8947 ELISA Kit; MIG10 ELISA Kit; pgk1 ELISA Kit; PGK1_HUMAN ELISA Kit; PGKA ELISA Kit; Phosphoglycerate kinase 1 ELISA Kit; Primer recognition protein 2 ELISA Kit; PRP 2 ELISA Kit
缩写：PGK1
Uniprot No.：P00558
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 94-105 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human PGK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-97 | ||||
| EDTA plasma (n=4) | 99 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.8943.0112.9532.7629002.2662.2442.2552.0644501.3931.4741.4341.2432250.7340.7630.7490.558112.50.4970.4800.4890.298560.3540.3380.3460.155280.2670.2770.2720.08100.1860.1950.191 | ||||||
货期：3-5 working days
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