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Rabbit Monoclonal SF3A3 Antibody

  • 中文名： SF3A3抗体
  • 别    名： SF3A3; PRP9; PRPF9; SAP61; SF3a60;;SF3A3
货号: IPDX19078
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC IHC:1/100-1/200;IHF:1/50-1/200 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSF3A3; PRP9; PRPF9; SAP61; SF3a60;;SF3A3
WB Predicted band size59 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human SF3A3
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于SF3A3抗体的3篇参考文献示例(内容基于典型研究主题，非真实文献)：

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1. **文献名称**：*SF3A3 is a core component of the U2 snRNP in spliceosome assembly*

**作者**：Will, C.L., Lührmann, R.

**摘要**：该研究通过免疫沉淀和质谱分析，证实SF3A3作为剪接体U2 snRNP的核心亚基，其抗体被用于验证其在pre-mRNA剪接中的关键作用，并揭示了SF3A3与其他剪接因子的相互作用网络。

2. **文献名称**：*Proteomic analysis of SF3A3-containing complexes in human cells*

**作者**：Hegele, A., et al.

**摘要**：利用SF3A3特异性抗体进行免疫共沉淀，结合蛋白质组学技术，系统鉴定了SF3A3在细胞核内的结合伙伴，揭示其在剪接调控和RNA加工中的多功能性。

3. **文献名称**：*Dysregulation of SF3A3 expression promotes tumorigenesis in colorectal cancer*

**作者**：Kfir, N., et al.

**摘要**：通过免疫组化(使用SF3A3抗体)和RNA干扰实验，证明SF3A3在结直肠癌中异常高表达，其缺失导致剪接错误和癌细胞凋亡，提示其作为潜在治疗靶点。

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注：以上文献为示例性质，实际研究中建议通过PubMed或Google Scholar以“SF3A3 antibody”及关联关键词检索真实文献。

背景信息

The SF3A3 antibody is a crucial tool for studying the SF3A complex, a core component of the U2 small nuclear ribonucleoprotein (snRNP) within the spliceosome. The spliceosome, a dynamic ribonucleoprotein machinery, catalyzes pre-mRNA splicing by removing introns and joining exons. SF3A3 (Splicing Factor 3A Subunit 3), along with SF3A1 and SF3A2. forms the SF3A heterotrimer, which stabilizes U2 snRNP binding to the branch site during spliceosome assembly. This interaction is essential for recognizing the intron's branchpoint sequence and ensuring splicing fidelity. SF3A3 antibodies are widely used in research to investigate spliceosome dynamics, RNA splicing mechanisms, and their dysregulation in diseases. They enable detection of SF3A3 expression levels via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in studies of splicing-related pathologies, including cancers and neurodegenerative disorders. Mutations or altered expression of SF3A3 have been linked to myelodysplastic syndromes and chemotherapy resistance, underscoring its clinical relevance. Additionally, these antibodies help screen for small molecules targeting the spliceosome, such as pladienolide derivatives, which inhibit SF3B complexes in cancer therapy. Validated for specificity, SF3A3 antibodies remain indispensable for dissecting splicing regulation and developing therapeutic strategies.

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