首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠前列环素(PGI2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PGI2含量。PGI2，即前列环素，是一种花生四烯酸的代谢产物，具有广泛的生物学功能，如血管舒张、抗血小板聚集和免疫调节等。其研究机制涉及与特定受体结合，激活G蛋白，进而调节细胞内信号传导。近年来，PGI2在治疗肺动脉高压等疾病中的应用研究取得了显著进展。试剂盒检测范围为0.25 ng/mL-50 ng/mL，适用于科研领域中对大鼠模型血管功能研究、抗血栓药物作用机制探索、炎症相关疾病动物实验的分子标志物检测，以及组织微环境中PGI2代谢动态分析，为心血管生物学、药理学及病理机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PGI2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.25 ng/mL-50 ng/mL
灵敏度：0.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of rat PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 500.2230.2220.223 12.50.3560.3510.354 40.5460.5570.552 10.7940.8040.799 0.251.1731.2751.224 02.4042.3052.355 | ||||||
货期：3-5 working days
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