首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前列环素(PGI2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的PGI2含量。PGI2即前列环素，是一种具有强大扩血管及抗血小板聚集作用的生物活性物质。可激活腺苷酸环化酶，升高血小板内cAMP水平，抑制血小板聚集并舒张血管，在心血管疾病等方面的防治研究上具有重要意义。试剂盒检测范围为50 pg/mL-2000 pg/mL，该产品适用于心血管机制研究、药物对血管内皮功能影响的体外评估，以及炎症或血栓性疾病模型中PGI2动态变化的定量分析，为探索血管调节机制、抗血小板药物开发等科研方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PGI2
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：50 pg/mL-2000 pg/mL
灵敏度：50 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Biochemicals
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGI2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 86-101 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 86-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 91-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of human PGI2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-98 | ||||
| EDTA plasma (n=4) | 96 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.6662.5172.592 501.2841.2351.260 1500.6250.6410.633 4600.3490.3650.357 10000.2210.2290.225 20000.1280.1320.130 | ||||||
货期：3-5 working days
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