首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胎盘生长因子(PLGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PGF含量。试剂盒检测范围为1.67 ng/mL-40 ng/mL，体外培养细胞模型、动物实验样本或临床前研究中的PLGF定量分析，生殖医学、肿瘤生物学及血管病理机制研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：D12S1900 ELISA Kit; Pgf ELISA Kit; PGFL ELISA Kit; PIGF ELISA Kit; Placenta growth factor ELISA Kit; Placental growth factor ELISA Kit; Placental growth factor; vascular endothelial growth factor related protein ELISA Kit; PlGF 2 ELISA Kit; PlGF ELISA Kit; PLGF_HUMAN ELISA Kit; PlGF2 ELISA Kit; SHGC 10760 ELISA Kit
缩写：PGF
Uniprot No.：P49763
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.67 ng/mL-40 ng/mL
灵敏度：0.7 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 83-97 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 89-101 | |||||
回收率：
| The recovery of human PLGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-100 | ||||
| EDTA plasma (n=4) | 95 | 91-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.0041.9861.9951.948 201.3161.3451.3311.284 100.7590.8170.7880.741 3.30.3530.3520.3530.306 1.670.2070.2190.2130.166 00.0460.0480.047 | ||||||
货期：3-5 working days
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