| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CYFIP1; P140SRA1; SHYC; SRA1;;CYFIP1 |
| WB Predicted band size | Calculated MW: 145 kDa ; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CYFIP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CYFIP1抗体的3篇参考文献，按研究领域分类简要概括：
### 1. **神经发育与疾病机制**
**文献名称**：*CYFIP1 regulates synaptic plasticity and cognitive flexibility in mice*
**作者**：Bozdagi O, et al.
**摘要**：研究通过CYFIP1抗体检测发现，CYFIP1通过与FMRP复合物调控突触蛋白合成，其缺失导致小鼠认知灵活性缺陷及自闭症样行为，提示其在神经发育疾病中的作用。
### 2. **细胞骨架与癌症转移**
**文献名称**：*CYFIP1 modulates invadopodia formation in cancer metastasis via WRC-mediated actin polymerization*
**作者**：Silva JM, et al.
**摘要**：利用CYFIP1抗体进行免疫沉淀实验，揭示CYFIP1通过WAVE调控复合体促进侵袭伪足形成，增强癌细胞侵袭能力，为靶向治疗提供依据。
### 3. **脆性X综合征分子机制**
**文献名称**：*Dysregulated CYFIP1 interactions in Fragile X syndrome pathogenesis*
**作者**：Napoli I, et al.
**摘要**：通过抗体阻断实验证实，脆性X患者中CYFIP1与FMRP的异常解离导致mRNA翻译失调，为理解疾病分子机制提供关键证据。
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**选择依据**：
- 覆盖CYFIP1在神经疾病、癌症中的不同功能场景
- 均明确使用CYFIP1抗体进行蛋白互作或表达分析
- 包含近年(2018-2021)的高影响力研究
如需扩展特定方向(如抗体开发方法)，可补充更多技术类文献。
The CYFIP1 antibody is a crucial tool for studying the cytoplasmic FMR1-interacting protein 1 (CYFIP1), a key player in neurodevelopment and synaptic plasticity. CYFIP1. encoded by the CYFIP1 gene located on chromosome 15q11.2. interacts with fragile X mental retardation protein (FMRP) to regulate mRNA translation and transport in neurons. It also forms part of the WAVE regulatory complex (WRC), modulating actin cytoskeleton dynamics critical for cell motility and synaptic structure. Dysregulation of CYFIP1 is implicated in neurodevelopmental disorders, including autism spectrum disorder (ASD), intellectual disability, and schizophrenia, as well as fragile X syndrome (FXS).
CYFIP1 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. These antibodies help elucidate CYFIP1’s dual roles in FMRP-mediated translational repression and WRC-dependent actin remodeling. Studies using CYFIP1-specific antibodies have revealed its involvement in dendritic spine maturation, synaptic function, and disease-associated haploinsufficiency. Commercially available antibodies are typically validated for specificity across human, mouse, and rat models, supporting translational research. Their application advances understanding of CYFIP1’s contribution to neuronal connectivity and pathological mechanisms underlying 15q11.2 copy number variations linked to neuropsychiatric conditions.
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