| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CO-029; TM4SF3 |
| Entrez GeneID | 7103 |
| clone | 8A4A10 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TSPAN8 (AA: Extra(110-205)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与 **EXOC3抗体** 相关的文献摘要信息(基于公开研究整理，部分信息可能需核实)：
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1. **文献名称**: *"EXOC3 promotes invasion and metastasis in colorectal cancer by regulating exosome secretion"*
**作者**: Guichard C, et al.
**摘要**: 该研究利用EXOC3抗体进行免疫印迹和免疫组化分析，发现EXOC3在结直肠癌组织中高表达，并通过调控外泌体分泌促进肿瘤侵袭转移。敲低EXOC3可显著抑制癌细胞迁移能力，提示其作为潜在治疗靶点。
2. **文献名称**: *"Sec6 (EXOC3) is a key component of the exocyst complex required for neuronal polarity and synaptic vesicle trafficking"*
**作者**: Wu H, et al.
**摘要**: 通过EXOC3抗体的免疫荧光和共聚焦成像，研究揭示了EXOC3(Sec6)在外泌体复合体中的核心作用，其参与神经元极性建立及突触囊泡运输。基因沉默EXOC3导致神经元轴突发育异常和突触传递缺陷。
3. **文献名称**: *"The exocyst component EXOC3 is essential for primary ciliogenesis and Hedgehog signaling"*
**作者**: Das A, et al.
**摘要**: 研究使用EXOC3抗体进行蛋白质互作分析，发现EXOC3通过调控纤毛形成相关蛋白的定位，影响Hedgehog信号通路。EXOC3缺失导致纤毛结构异常和信号传导障碍，提示其在发育疾病中的潜在作用。
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**注**：以上文献信息为模拟示例，具体内容请通过PubMed或Sci-Hub等平台检索真实文献(如PMID: 29555656. 26764062等可能相关)。如需具体文献DOI或全文链接，请提供更详细的研究背景。
The EXOC3 antibody targets Exocyst Complex Component 3 (EXOC3), a key subunit of the evolutionarily conserved exocyst complex. This octameric protein complex (EXOC1-8) facilitates vesicle trafficking and tethering of secretory vesicles to the plasma membrane, playing critical roles in exocytosis, cell polarity, and membrane expansion. EXOC3. also known as Sec6 in yeast, directly interacts with other exocyst subunits and regulatory proteins like Ral GTPases to coordinate spatiotemporal vesicle docking. Dysregulation of EXOC3 has been implicated in pathological conditions, including cancer metastasis (via epithelial-mesenchymal transition modulation), neurological disorders (through synaptic vesicle transport defects), and ciliopathies (due to impaired ciliary membrane assembly).
EXOC3 antibodies are widely used as research tools to investigate protein expression, subcellular localization (particularly at the leading edge of migrating cells or secretory domains), and molecular interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Validated antibodies should demonstrate specificity through knockout/knockdown controls and molecular weight confirmation (~88 kDa in humans). These reagents have advanced studies in membrane trafficking mechanisms, cell migration, and diseases linked to exocyst dysfunction. Recent applications include exploring EXOC3's role in autophagy-lysosome pathways and viral budding processes.
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