首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠前列腺素E2(PGE2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PG-E2含量。PG-E2即前列腺素E2，它在体内广泛存在，参与多种生理和病理过程。在炎症、免疫调节、肿瘤发生发展等方面扮演重要角色。研究机制主要围绕其与受体结合，激活多条信号通路，影响细胞增殖、分化和炎症因子释放等过程。试剂盒检测范围为0.5 pg/mL-100 pg/mL，适用于体外实验研究，例如探究炎症模型中PGE2的动态变化、评估药物或化合物对前列腺素代谢通路的影响，以及组织微环境中脂质介质的定量分析等科研场景，为炎症机制研究、药效学评价及生物标志物探索提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PG-E2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.5 pg/mL-100 pg/mL
灵敏度：0.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 99-109 | |||||
回收率：
| The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-99 | ||||
| EDTA plasma (n=4) | 101 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 1000.2280.2240.226 250.3650.3480.357 80.5600.5390.550 20.8130.7870.800 0.51.2581.2341.246 02.0312.1762.104 | ||||||
货期：3-5 working days
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