| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC8; Dtymk; TMPK; TYMK; ;DTYMK |
| WB Predicted band size | Calculated MW: 24 kDa ; Observed MW: 23 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human DTYMK |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于DTYMK抗体的示例参考文献(注：以下内容为假设性示例，建议通过学术数据库检索真实文献)：
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1. **文献名称**: "Characterization of a Novel DTYMK-Specific Antibody for Detecting Thymidylate Kinase Activity in Cancer Cells"
**作者**: Zhang L, et al.
**摘要**: 本研究开发并验证了一种高特异性DTYMK抗体，用于检测多种癌细胞系中胸苷酸激酶的活性。通过Western blot和免疫荧光验证了抗体在结直肠癌和乳腺癌模型中的适用性，发现DTYMK表达与癌细胞增殖速率正相关。
2. **文献名称**: "DTYMK as a Prognostic Marker in Glioblastoma: Immunohistochemical Analysis Using a Clinical-Grade Antibody"
**作者**: Smith JR, et al.
**摘要**: 利用商业化DTYMK抗体对胶质母细胞瘤组织进行免疫组化分析，发现DTYMK高表达与患者总生存期缩短显著相关，提示其可作为潜在预后标志物及治疗靶点。
3. **文献名称**: "Functional Role of DTYMK in DNA Repair Pathways Revealed by Antibody-Mediated Knockdown Studies"
**作者**: Tanaka K, et al.
**摘要**: 通过抗体介导的蛋白质敲低技术，研究DTYMK在DNA损伤修复中的作用。实验表明，抑制DTYMK会导致DNA复制应激增强，并影响同源重组修复效率。
4. **文献名称**: "Validation of a Polyclonal DTYMK Antibody for Flow Cytometry Applications in Hematopoietic Stem Cells"
**作者**: Müller S, et al.
**摘要**: 本研究验证了一种多克隆DTYMK抗体在流式细胞术中的应用，证实其在造血干细胞中可特异性识别DTYMK蛋白，并用于分析细胞周期依赖性表达变化。
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**建议**：如需真实文献，可通过PubMed或Google Scholar检索关键词(如“DTYMK antibody validation”“DTYMK immunohistochemistry”)，并筛选近年发表的抗体应用类研究。
The DTYMK antibody targets deoxythymidylate kinase (DTYMK), a key enzyme in the nucleotide biosynthesis pathway. DTYMK, also known as thymidylate kinase, catalyzes the phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), a critical step in synthesizing dTTP for DNA replication and repair. This enzyme is highly expressed during the S phase of the cell cycle and is essential for proliferating cells, particularly in rapidly dividing tissues and cancers.
DTYMK antibodies are widely used in research to study protein expression, localization, and function in cellular models. They enable detection of DTYMK via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding investigations into cell proliferation, cancer biology, and genomic stability. Elevated DTYMK levels are associated with tumor progression, making it a potential biomarker or therapeutic target in oncology.
Recent studies also link DTYMK dysregulation to neurodegenerative disorders, such as Alzheimer’s disease, where impaired nucleotide metabolism may contribute to neuronal dysfunction. Additionally, structural and functional analyses using DTYMK antibodies help elucidate its enzymatic mechanisms and interactions with other proteins or inhibitors.
Overall, DTYMK antibodies serve as vital tools for exploring roles of thymidylate metabolism in health and disease, bridging basic research with translational applications in cancer therapy and beyond.
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