首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子前列腺素E2(PGE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PG-E2含量。PG - E2即前列腺素 E2，是一种具有广泛生理和病理作用的脂质介质。它参与炎症反应、免疫调节、细胞增殖等多种生理过程。在研究机制上，通过与特定受体结合激活下游信号通路，在肿瘤、炎症性疾病等领域是热点研究对象。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，可广泛应用于实验动物模型中炎症动态监测、药理学研究中的化合物效能评估，以及体外细胞实验中炎症因子调控机制的探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PG-E2
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 80-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 85-110 | ||||
| EDTA plasma (n=4) | 90 | 80-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.9132.8122.8632.70310002.1382.0382.0881.9285001.1571.0981.1280.9682500.6150.5980.6070.4471250.3720.3470.3600.20062.50.2820.2720.2770.11731.250.2030.1870.1950.03500.1630.1570.160 | ||||||
货期：3-5 working days
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