首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人前列腺素D2(PGD2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、组织匀浆、尿液样本中的PGD2含量。PGD2即前列腺素D2，是一种重要的脂质介质。它参与多种生理病理过程，如炎症反应、睡眠调节等。在研究机制上，PGD2通过与特定受体结合，激活下游信号通路，调控细胞的功能和基因表达，是炎症相关疾病研究的热门靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品可广泛应用于炎症性疾病机制研究、药物筛选模型中的PGD2动态监测、泌尿系统相关科研中的生物标志物分析，以及代谢组学研究中的脂质介质定量检测，为体外实验和动物模型研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PGD2
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, tissue homogenates, urine
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGD2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-110 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human PGD2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15001.9851.8541.9201.8407501.4031.3231.3631.2833750.9120.8860.8990.819187.50.5450.5250.5350.455940.3710.3590.3650.285470.2040.1980.2010.12123.50.1290.1260.1280.04800.0800.0790.080 | ||||||
货期：3-5 working days
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