| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARA54; HFB30; Rnf14; TRIAD2;;RNF14 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RNF14 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于RNF14抗体的3篇参考文献的简要概括(文献信息为模拟示例)：
1. **文献名称**：RNF14 regulates androgen receptor signaling in prostate cancer
**作者**：Smith A, et al.
**摘要**：研究利用RNF14抗体进行免疫共沉淀(Co-IP)实验，发现RNF14通过泛素化修饰调控雄激素受体(AR)的稳定性，促进前列腺癌细胞增殖。
2. **文献名称**：RNF14 interacts with Huntingtin and modulates polyglutamine toxicity
**作者**：Lee J, et al.
**摘要**：通过Western blot和免疫荧光(使用RNF14特异性抗体)，发现RNF14与突变亨廷顿蛋白结合，影响其聚集和神经细胞毒性，提示其在神经退行性疾病中的作用。
3. **文献名称**：The E3 ligase RNF14 promotes DNA damage repair via ubiquitination of BRCA1
**作者**：Wang Y, et al.
**摘要**：利用RNF14抗体进行染色质免疫沉淀(ChIP)分析，揭示RNF14通过泛素化BRCA1调控DNA损伤修复通路，影响乳腺癌细胞对放化疗的敏感性。
(注：以上文献信息为基于领域知识的模拟生成，实际引用需查询具体数据库。)
The RNF14 antibody is a crucial tool for studying the Ring Finger Protein 14 (RNF14), an E3 ubiquitin ligase involved in ubiquitination and protein degradation pathways. RNF14. encoded by the *RNF14* gene in humans, contains a characteristic RING finger domain critical for its enzymatic activity in tagging target proteins with ubiquitin for proteasomal degradation. It plays roles in diverse cellular processes, including transcriptional regulation, DNA repair, and metabolic signaling. RNF14 interacts with androgen receptors (AR), modulating their stability and activity, which links it to prostate cancer research. Additionally, it has been implicated in neurodegenerative diseases and metabolic disorders due to its involvement in protein homeostasis and stress response pathways.
RNF14 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides derived from specific regions of the RNF14 protein. These antibodies are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to detect RNF14 expression, localization, and protein interactions. High-quality RNF14 antibodies exhibit specificity for distinguishing RNF14 from homologous proteins like RNF8 or RNF4. Researchers rely on these antibodies to explore RNF14's functional roles in disease models, therapeutic target validation, and biomarker discovery, particularly in cancer and metabolic syndrome studies. Proper validation via knockout controls or siRNA knockdown is essential to confirm antibody reliability.
×
