| WB | 咨询技术 | Mouse,Rat |
| IF | 咨询技术 | Mouse,Rat |
| IHC | 咨询技术 | Mouse,Rat |
| ICC | 技术咨询 | Mouse,Rat |
| FCM | 咨询技术 | Mouse,Rat |
| Elisa | 咨询技术 | Mouse,Rat |
| Aliases | N4BP1;;N4BP1 |
| WB Predicted band size | Calculated MW: 99 kDa ; Observed MW: 110 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse,Rat |
| Immunogen | A synthesized peptide derived from mouse N4BP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于N4BP1抗体的3篇参考文献示例(内容为模拟，仅供参考)：
1. **文献名称**：*N4BP1 regulates antiviral immunity by suppressing virus-induced NF-κB activation*
**作者**：Li, Y., et al.
**摘要**：本研究利用N4BP1特异性抗体进行免疫共沉淀和Western blot分析，发现N4BP1通过抑制病毒诱导的NF-κB信号通路，负调控宿主抗病毒免疫反应。
2. **文献名称**：*N4BP1 interacts with the E3 ligase TRIM21 to promote inflammatory signaling*
**作者**：Wang, Q., et al.
**摘要**：通过免疫荧光和免疫组化实验(使用N4BP1抗体)，作者揭示N4BP1与TRIM21的相互作用可增强炎症因子的释放，参与自身免疫疾病的发病机制。
3. **文献名称**：*N4BP1 loss promotes genomic instability in colorectal cancer*
**作者**：Garcia-Sanchez, A., et al.
**摘要**：研究通过N4BP1抗体检测结直肠癌组织中的蛋白表达水平，发现N4BP1缺失导致DNA修复缺陷，与患者不良预后相关。
4. **文献名称**：*Structural and functional analysis of N4BP1 in RNA metabolism*
**作者**：Nguyen, T., et al.
**摘要**：利用N4BP1抗体进行质谱分析，鉴定其与RNA结合蛋白的相互作用网络，表明N4BP1在RNA稳定性调控中发挥重要作用。
(注：以上文献为示例，具体内容需根据实际检索结果调整。)
**Background of N4BP1 Antibody**
N4BP1 (NEDD4-binding protein 1) is an evolutionarily conserved protein implicated in regulating immune responses, antiviral defense, and cellular signaling pathways. It interacts with NEDD4. an E3 ubiquitin ligase, to modulate ubiquitination-dependent processes such as protein degradation, receptor trafficking, and immune regulation. Structurally, N4BP1 contains a PB1 domain for protein interactions and a ubiquitin-associated (UBA) domain, suggesting roles in ubiquitin signaling.
Studies highlight N4BP1's involvement in limiting excessive inflammation by suppressing NF-κB and type I interferon pathways, making it a critical checkpoint in antiviral immunity. Dysregulation of N4BP1 has been linked to autoimmune disorders and cancer, with evidence showing its tumor-suppressive role in certain contexts.
N4BP1 antibodies are essential tools for detecting the protein’s expression, localization, and interactions in research. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study its function in immune regulation, viral infection responses, and cancer biology. These antibodies (often rabbit or mouse-derived) enable insights into N4BP1's molecular mechanisms and therapeutic potential, though clinical applications remain exploratory.
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