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产品描述：人磷酸甘油酸变位酶2(PGAM2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PGAM2含量。PGAM2是糖酵解途径中的关键酶，与心肌肥厚和心力衰竭相关。研究显示，PGAM2在心肌肥厚向心衰进展中起重要作用，其详细功能尚不明确，可能通过调节能量代谢和氧化应激等途径影响心脏功能。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于基础科研领域，如研究骨骼肌代谢机制、红细胞能量代谢动态、代谢相关疾病动物模型中的PGAM2表达水平变化，以及体外培养细胞中糖酵解途径的调控分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BPG dependent PGAM 2 ELISA Kit; BPG-dependent PGAM 2 ELISA Kit; GSD10 ELISA Kit; MGC88743 ELISA Kit; Muscle specific phosphoglycerate mutase ELISA Kit; Muscle-specific phosphoglycerate mutase ELISA Kit; OTTHUMP00000207787 ELISA Kit; PGAM 2 ELISA Kit; PGAM M ELISA Kit; PGAM-M ELISA Kit; Pgam2 ELISA Kit; PGAM2_HUMAN ELISA Kit; PGAMM ELISA Kit; phosphoglycerate mutase 2 (muscle) ELISA Kit; Phosphoglycerate mutase 2 ELISA Kit; Phosphoglycerate mutase isozyme M ELISA Kit
缩写：PGAM2
Uniprot No.：P15259
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 98-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human PGAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.5262.4172.4722.327 5001.6081.5051.5571.412 2500.8840.8550.8700.725 1250.4860.4940.4900.345 62.50.3290.3060.3180.173 31.20.2650.2570.2610.116 15.60.1970.1890.1930.048 00.1450.1440.145 | ||||||
货期：3-5 working days
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