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产品描述：人PRKC凋亡WT1调节蛋白(PAWR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PAWR含量。PAWR 即 PRKC, Apoptosis, WT1, Regulator，是一种肿瘤抑制基因。它能通过多种信号通路调节细胞凋亡、增殖与分化。研究发现其表达异常与肿瘤发生发展密切相关，深入研究其作用机制，有望为癌症等疾病的治疗提供新靶点和策略。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于肿瘤生物学研究、细胞凋亡机制探索、药物作用靶点筛选等科研领域，尤其适用于PAWR相关信号通路的功能研究及其在疾病模型中的表达水平动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2310001G03Rik ELISA Kit; PAR 4 ELISA Kit; PAR-4 ELISA Kit; Pawr ELISA Kit; PAWR_HUMAN ELISA Kit; PRKC Apoptosis WT1 Regulator ELISA Kit; PRKC apoptosis WT1 regulator protein ELISA Kit; Prostate apoptosis response 4 protein ELISA Kit; Prostate apoptosis response protein 4 ELISA Kit; prostate apoptosis response protein PAR-4 ELISA Kit; Transcriptional repressor Par-4-like protein PAWR ELISA Kit; Transcriptional repressor PAR4 ELISA Kit; WT1 Interacting Protein ELISA Kit
缩写：PAWR
Uniprot No.：Q96IZ0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAWR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-91 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 84-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-101 | |||||
回收率：
| The recovery of human PAWR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-102 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.7452.6522.6992.5698002.2462.1762.2112.0814001.7611.7041.7331.6032001.1671.1391.1531.0231000.5240.5690.5470.417500.3120.3090.3110.181250.1980.1890.1940.06400.1270.1330.130 | ||||||
货期：3-5 working days
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