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产品描述:人妊娠相关血浆蛋白A(PAPP-A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、尿液样本中的PAPPA含量。PAPPA即妊娠相关血浆蛋白A,在心血管疾病、肿瘤等领域有重要作用。其背景为可作为一些疾病生物标志物。研究机制上,PAPPA可裂解胰岛素样生长因子结合蛋白,调节胰岛素样生长因子活性,进而影响细胞增殖、迁移等生物学过程。试剂盒检测范围为1.7 ng/mL-50 ng/mL,适用于体外分析不同生理或病理条件下PAPP - A的表达动态,可支持生殖医学、胎盘功能机制研究、妊娠相关疾病模型评估等科研项目,同时也可用于探索PAPP - A在心血管疾病、肿瘤微环境等跨学科领域中的潜在生物学功能本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:ASBABP2 ELISA Kit; Aspecific BCL2 ARE binding protein 2 ELISA Kit; Differentially placenta 1 expressed protein ELISA Kit; DIPLA1 ELISA Kit; EC 3.4.24.79 ELISA Kit; IGF dependent IGFBP 4 protease ELISA Kit; IGF-dependent IGFBP-4 protease ELISA Kit; IGFBP 4ase ELISA Kit; IGFBP-4ase ELISA Kit; IGFBP4ase ELISA Kit; Insulin like growth factor dependent IGF binding protein 4 protease ELISA Kit; Insulin-like growth factor-dependent IGF-binding protein 4 protease ELISA Kit; PAPA ELISA Kit; PAPP A ELISA Kit; PAPP-A ELISA Kit; PAPP1_HUMAN ELISA Kit; PAPPA ELISA Kit; PAPPA1 ELISA Kit; Pappalysin 1 precursor ELISA Kit; Pappalysin-1 ELISA Kit; Pregnancy Associated Plasma Protein A ELISA Kit; Pregnancy associated plasma protein A pappalysin 1 ELISA Kit; Pregnancy-associated plasma protein A ELISA Kit
缩写:PAPPA
Uniprot No.:Q13219
种属:Homo sapiens (Human)
样本类型:serum, plasma, urine
检测范围:1.7 ng/mL-50 ng/mL
灵敏度:0.4 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cell Biology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PAPP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:5 | Average % | 86 | ||||
Range % | 80-93 | |||||
1:10 | Average % | 90 | ||||
Range % | 85-95 | |||||
1:20 | Average % | 95 | ||||
Range % | 90-100 | |||||
1:40 | Average % | 94 | ||||
Range % | 86-100 |
回收率:
The recovery of human PAPP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 502.5422.4522.4972.450 20.81.2831.3561.3201.273 8.30.6420.6310.6370.590 3.30.2870.3050.2960.249 1.70.1550.1600.1580.111 00.0460.0470.047 |
货期:3-5 working days
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