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产品描述：人蛋白 DJ-1 (PARK7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆、细胞裂解物样本中的PARK7含量。PARK7是一个重要靶点。它与帕金森病等神经系统疾病相关。研究表明，PARK7具有抗氧化应激、分子伴侣等功能，其表达异常会影响细胞内信号通路，干扰神经细胞正常生理功能，目前对其在疾病发生发展中的机制探索是研究热点。试剂盒检测范围为0.235 ng/mL-15 ng/mL，该产品适用于神经退行性疾病机制研究、氧化应激相关实验模型验证、药物干预效果评价等科研场景，为探索DJ - 1在疾病发生发展中的分子作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CAP1 ELISA Kit; DJ-1 ELISA Kit; DJ1 ELISA Kit; DJ1 protein ELISA Kit; Epididymis secretory sperm binding protein Li 67p ELISA Kit; FLJ27376 ELISA Kit; FLJ34360 ELISA Kit; FLJ92274 ELISA Kit; HEL S 67p ELISA Kit; Oncogene DJ1 ELISA Kit; OTTHUMP00000001348 ELISA Kit; OTTHUMP00000001349 ELISA Kit; OTTHUMP00000001350 ELISA Kit; OTTHUMP00000001351 ELISA Kit; PARK7 ELISA Kit; PARK7_HUMAN ELISA Kit; Parkinson disease (autosomal recessive, early onset) 7 ELISA Kit; Parkinson disease protein 7 ELISA Kit; Parkinson protein 7 ELISA Kit; Protein DJ-1 ELISA Kit; SP22 ELISA Kit
缩写：PARK7
Uniprot No.：Q99497
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates, cell lysates
检测范围：0.235 ng/mL-15 ng/mL
灵敏度：0.058 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PARK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human PARK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected151.8451.7521.7991.6007.51.5321.4761.5041.3053.751.1121.1041.1080.9091.880.7840.7390.7620.5630.940.4650.4340.4500.2510.470.3760.3590.3680.1690.2350.2930.2900.2920.09300.2010.1970.199 | ||||||
货期：3-5 working days
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