首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人冠毛素-2(PAPPA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的PAPPA2含量。PAPPA2是一个有研究价值的靶点。其在多种生理与病理过程中可能发挥作用。研究机制上，主要聚焦于它在细胞增殖、分化及肿瘤发生发展等方面的影响，探索其信号通路及相关分子作用方式，以寻找潜在治疗干预点。试剂盒检测范围为2 ng/mL-60 ng/mL，适用于基础医学研究领域，如探索PAPPA2在胚胎发育、肿瘤微环境调控或代谢性疾病模型中的作用机制，也可用于动物实验中生物标志物动态变化的监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PAPPA 2 ELISA Kit; PAPP E ELISA Kit; PAPP-A2 ELISA Kit; PAPP-E ELISA Kit; PAPP2_HUMAN ELISA Kit; PAPPA2 ELISA Kit; Pappalysin 2 ELISA Kit; Pappalysin-2 ELISA Kit; PLAC3 ELISA Kit; Pregnancy associated plasma protein A2 ELISA Kit; Pregnancy associated plasma protein E1 ELISA Kit; Pregnancy-associated plasma protein A2 ELISA Kit; Pregnancy-associated plasma protein E1 ELISA Kit
缩写：PAPPA2
Uniprot No.：Q9BXP8
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：2 ng/mL-60 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAPPA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-98 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-99 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-101 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 86-102 | ||||||
回收率：
| The recovery of human PAPPA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-100 | |||||
| EDTA plasma (n=4) | 95 | 88-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 602.5172.4832.5002.453 251.2941.3281.3111.264 100.6140.6260.6200.573 40.3110.2990.3050.258 20.1590.1560.1580.111 00.0460.0470.047 | |||||||
货期：3-5 working days
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