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产品描述：人多聚ADP核糖聚合酶(PARP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PARP1含量。PARP1是重要的DNA损伤修复酶，在维持基因组稳定性等方面发挥作用。其背景涉及DNA损伤引发的细胞生理反应。研究机制主要围绕其对DNA单链断裂修复，通过催化多聚ADP - 核糖基化反应招募修复蛋白，抑制它可使肿瘤细胞DNA损伤累积致死亡。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于药物开发中PARP抑制剂作用评估、细胞应激反应研究或疾病模型中DNA损伤修复机制的探索；可满足分子生物学、肿瘤学等领域的实验需求，为探索PARP相关信号通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) ELISA Kit; ADP ribosyltransferase ELISA Kit; ADP ribosyltransferase diphtheria toxin like 1 ELISA Kit; ADP ribosyltransferase NAD(+) ELISA Kit; ADPRT 1 ELISA Kit; ADPRT ELISA Kit; ADPRT1 ELISA Kit; ARTD1 ELISA Kit; msPARP ELISA Kit; NAD(+) ADP ribosyltransferase 1 ELISA Kit; NAD(+) ADP-ribosyltransferase 1 ELISA Kit; pADPRT 1 ELISA Kit; pADPRT-1 ELISA Kit; pADPRT1 ELISA Kit; PARP 1 ELISA Kit; PARP ELISA Kit; PARP-1 ELISA Kit; PARP1 ELISA Kit; PARP1_HUMAN ELISA Kit; Poly (ADP ribose) polymerase 1 ELISA Kit; poly (ADP ribose) polymerase family; member 1 ELISA Kit; Poly (ADP-ribose) polymerase 1 ELISA Kit; Poly [ADP-ribose] polymerase 1 ELISA Kit; Poly(ADP ribose) polymerase ELISA Kit; poly(ADP ribose) synthetase ELISA Kit; poly(ADP ribosyl)transferase ELISA Kit; Poly(ADP-ribosyl)transferase ELISA Kit; Poly[ADP ribose] synthetase 1 ELISA Kit; Poly[ADP-ribose] synthase 1 ELISA Kit; PPOL ELISA Kit; sPARP 1 ELISA Kit; sPARP1 ELISA Kit
缩写：PARP1
Uniprot No.：P09874
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PARP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human PARP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 97-105 | ||||
| EDTA plasma (n=4) | 92 | 86-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3012.3692.3352.1885001.5821.5981.5901.4432500.9480.9680.9580.8111250.5780.5850.5820.43562.50.3890.3680.3790.23231.250.2870.2640.2760.12915.60.2050.2130.2090.06200.1470.1460.147 | ||||||
货期：3-5 working days
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