首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子纤溶酶原激活物抑制因子1(PAI-1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PAI-1含量。PAI - 1即纤溶酶原激活物抑制物 - 1，它在体内参与纤溶系统的平衡调节。其异常表达与多种疾病如血栓形成、肿瘤转移等密切相关。研究机制主要围绕调控其活性及表达，以改善纤溶功能、影响疾病进程，为相关疾病治疗提供思路。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于心血管疾病模型、器官纤维化机制研究、药物干预效果评估等科研场景，为探究PAI - 1在病理生理过程中的动态变化提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PAI-1
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:40 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:80 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:160 | Average % | 90 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of rabbit PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 80-86 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average4000.1240.1260.1252000.2060.2150.2111000.3680.3500.359500.5450.5710.558250.7680.7650.76712.50.9170.9380.9286.251.1281.1571.14301.5861.5991.593 | ||||||
货期：3-5 working days
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