首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人纤溶酶抗纤溶酶复合物(PAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液样本中的PAP含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可为研究血栓形成与溶解的动态平衡、评估抗凝药物作用机制或探索纤溶相关疾病的生物标志物提供可靠工具，例如在心血管疾病模型、肿瘤微环境分析或炎症相关实验中进行样本内源性PAP水平的精准测定。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：PAP
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 100 | ||||
| Range % | 91-108 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 92-109 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-101 | ||||
| EDTA plasma (n=4) | 93 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.8192.8152.8172.68852.2442.2502.2472.1182.51.2911.3011.2961.1671.250.7300.7930.7620.6330.6250.4430.4530.4480.3190.3120.2310.2500.2410.1120.1560.1570.1680.1630.03400.1240.1340.129 | ||||||
货期：3-5 working days
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