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产品描述：人P2X嘌呤受体7(P2RX7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的P2RX7含量。P2RX7是一种配体门控离子通道型嘌呤受体。其在免疫调节、神经炎症等生理和病理过程中发挥作用。研究机制上，它可被细胞外ATP激活，参与细胞因子释放、细胞凋亡等过程，是治疗炎症性疾病、神经退行性疾病等潜在靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，为研究其在信号通路激活、细胞焦亡机制或病理模型中的功能提供可靠工具；尤其适用于探索炎症相关疾病机制、药物靶点筛选或生物标志物分析的科研场景；满足实验室对复杂样本的高通量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ATP receptor ELISA Kit; P2rx7 ELISA Kit; P2RX7_HUMAN ELISA Kit; P2X purinoceptor 7 ELISA Kit; P2X7 ELISA Kit; P2Z receptor ELISA Kit; Purinergic receptor ELISA Kit; purinergic receptor P2X, ligand gated ion channel, 7 ELISA Kit
缩写：P2RX7
Uniprot No.：Q99572
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human P2RX7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-104 | |||||
回收率：
| The recovery of human P2RX7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 89-104 | ||||
| EDTA plasma (n=4) | 96 | 89-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.0161.8981.9571.8718001.1521.1151.1341.0484000.6770.6470.6620.5762000.3630.3740.3690.2831000.2410.2520.2470.161500.1880.2000.1940.108250.1270.1340.1310.04500.0850.0870.086 | ||||||
货期：3-5 working days
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