首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠氧化低密度脂蛋白(OxLDL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的OxLDL含量。OxLDL即氧化低密度脂蛋白，是体内低密度脂蛋白被氧化修饰形成。它在动脉粥样硬化等疾病发展中扮演重要角色。研究显示，OxLDL可被巨噬细胞摄取形成泡沫细胞，还能引发炎症反应、诱导细胞凋亡等，是心血管疾病研究的重要靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品适用于心血管疾病动物模型研究、药物干预效果评估、脂质代谢相关机制探索及细胞氧化应激实验等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：OxLDL
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 96-106 | |||||
| 1:800 | Average % | 90 | ||||
| Range % | 85-96 | |||||
回收率：
| The recovery of rat OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-100 | ||||
| EDTA plasma (n=4) | 91 | 89-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.6682.7832.7262.5412002.2962.2842.2902.1051001.7041.7351.7201.535501.0761.0831.0800.895250.6070.6240.6160.43112.50.4080.4170.4130.2286.250.2910.2980.2950.11000.1840.1860.185 | ||||||
货期：3-5 working days
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