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产品描述：兔子Ⅰ型前胶原N端前肽(PⅠNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PⅠNP含量。PⅠNP是一种重要靶点。它与骨代谢密切相关，是I型前胶原氨基端前肽。研究其机制主要聚焦在它反映成骨细胞活性和骨形成情况的作用上，通过检测它可辅助诊断骨质疏松症等骨病，在骨健康研究和临床诊断中有重要意义。试剂盒检测范围为0.78 ng/mL-50 ng/mL，该产品可广泛应用于骨代谢机制研究、骨骼疾病动物模型评估、骨再生药物开发等科研领域，为骨生物学、骨组织工程及代谢性骨病的实验研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：procollagen 1 N-terminal peptide(P1NP),_x000D_ procollagen I N-terminal peptide(PINP)
缩写：PⅠNP
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of rabbit PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 89 | 82-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.8432.7632.8032.721 252.3042.1282.2162.134 12.51.5771.4751.5261.444 6.250.9090.8990.9040.822 3.120.4260.4210.4240.342 1.560.2130.2060.2100.128 0.780.1470.1400.1440.062 00.0840.0800.082 | ||||||
货期：3-5 working days
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