首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅰ型前胶原羧基端肽(PⅠCP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的PⅠCP含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，可应用于骨骼发育研究、纤维化疾病模型评估、骨代谢相关药物筛选等科研领域，为探究骨形成活性、组织修复机制及病理状态下的胶原合成动态提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
缩写：PⅠCP
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of rat PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-89 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 2002.1912.2052.1982.026 1001.5781.6111.5951.423 500.9120.9310.9220.750 250.6030.6250.6140.442 12.50.4160.4050.4110.239 6.250.3190.2970.3080.136 3.120.2440.2410.2430.071 00.1730.1700.172 | ||||||
货期：3-5 working days
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