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产品描述：人Ⅰ型前胶原N端前肽(PⅠNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PⅠNP含量。PⅠNP即I型前胶原氨基端前肽，它是反映成骨细胞活性和骨形成的特异性指标。其背景在于可用于评估骨代谢状态。研究机制上，主要是通过检测血液中PⅠNP水平，来反映骨合成代谢过程，辅助诊断和监测骨质疏松症等骨病。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于骨组织工程研究、骨骼疾病动物模型评估、骨代谢相关分子机制解析等科研场景，为骨代谢研究、抗骨质疏松药物筛选及骨再生材料开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
缩写：PⅠNP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 89-102 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 83-97 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 94-105 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-99 | ||||
| EDTA plasma (n=4) | 101 | 95-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 12002.1912.2872.2392.086 6001.8851.8961.8911.738 3001.5271.5071.5171.364 1501.0011.0421.0220.869 750.6920.6480.6700.517 37.50.4990.4720.4860.333 18.750.2720.2880.2800.127 00.1520.1540.153 | ||||||
货期：3-5 working days
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