首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子氧化低密度脂蛋白(OxLDL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OxLDL含量。OxLDL 即氧化低密度脂蛋白，是低密度脂蛋白被氧化修饰的产物。它在动脉粥样硬化等疾病发生发展中起重要作用。研究机制方面，OxLDL 可被巨噬细胞摄取形成泡沫细胞，还能诱导炎症反应、促进血管平滑肌细胞增殖迁移，加速斑块形成。试剂盒检测范围为0.078 μmol/mL-5 μmol/mL，动脉粥样硬化动物模型构建、降脂药物作用机制研究、代谢综合征相关氧化应激评估、心血管病理机制探索、抗氧化治疗方案开发本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：OxLDL
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.078 μmol/mL-5 μmol/mL
灵敏度：0.02 μmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μmol/mlOD1OD2AverageCorrected52.6462.6062.6262.5482.52.1272.1132.1202.0421.251.4531.3451.3991.3210.6250.8320.8270.8300.7520.3120.4310.4240.4280.3500.1560.2340.2220.2280.1500.0780.1430.1330.1380.06000.0800.0760.078 | ||||||
货期：3-5 working days
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