首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人氧化低密度脂蛋白(OxLDL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、尿液、唾液、组织匀浆、细胞裂解物样本中的OxLDL含量。OxLDL即氧化型低密度脂蛋白，是一种被氧化修饰的脂蛋白。它与动脉粥样硬化等疾病密切相关。其研究机制主要聚焦在它可被巨噬细胞摄取形成泡沫细胞，还能引发炎症反应，损伤血管内皮细胞，促进动脉粥样硬化斑块形成。试剂盒检测范围为1.56 mU/mL-100 mU/mL，本试剂盒可应用于心血管疾病基础研究、氧化应激相关代谢机制探索、药物或天然产物抗氧化效价评估等科研领域，为体外实验提供高灵敏度的检测方案，但不适用于临床诊断。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：OxLDL
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, saliva, tissue homogenates, cell lysates
检测范围：1.56 mU/mL-100 mU/mL
灵敏度：0.78 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 93-105 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:800 | Average % | 86 | ||||
| Range % | 80-92 | |||||
回收率：
| The recovery of human OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-104 | ||||
| EDTA plasma (n=4) | 94 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2Average 1000.1300.1290.130 500.3060.3010.304 250.5490.5570.553 12.50.9410.9560.949 6.251.4011.4181.410 3.121.9531.9671.960 1.562.1862.0872.137 02.7582.6562.707 | ||||||
货期：3-5 working days
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