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Human oxidized high density lipoprotein (Ox-HDL)ELISA Kit

  • 中文名： 人氧化高密度脂蛋白(Ox-HDL)ELISA Kit
货号: IPD-E16552h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人氧化高密度脂蛋白(Ox-HDL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的Ox-HDL含量。Ox - HDL指氧化型高密度脂蛋白，HDL本具抗动脉粥样硬化等有益作用，但氧化后功能改变。研究机制上，Ox - HDL可通过多种途径影响细胞功能，比如促使巨噬细胞摄取脂质形成泡沫细胞，参与动脉粥样硬化等疾病的发生发展。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，可灵活应用于基础医学研究领域，如探究氧化应激状态下脂蛋白代谢机制、评估抗氧化药物干预效果、建立疾病模型生物标志物分析体系等；满足细胞实验、动物模型及临床前研究中对Ox-HDL的精准定量需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    Ox-HDL
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, urine, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%





    Three samples of known concentration were tested twenty times on one plate to assess.





    Inter-assay Precision (Precision between assays): CV%<10%





    Three samples of known concentration were tested in twenty assays to assess.












  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Ox-HDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.



    		SampleSerum(n=4)
    1:100Average %90
    Range %80-98

    1:200Average %96
    Range %91-100

    1:400Average %94
    Range %90-105

    1:800Average %92
    Range %86-98

  • 回收率：
    The recovery of human Ox-HDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.












    Sample TypeAverage % RecoveryRange



    Serum (n=5)9280-100



    EDTA plasma (n=4)9080-98

















  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected 20002.8132.7222.7682.607 10002.1222.0362.0791.918 5001.1541.1981.1761.015 2500.6230.5950.6090.448 1250.3680.3470.3580.197 62.50.2920.2720.2820.121 31.250.2050.1920.1990.038 00.1640.1580.161　 

  • 货期：
    3-5 working days

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